Difference between revisions of "R Hackathon 1/PGLS"
Revision as of 15:18, 6 February 2008
Phylogenetic Generalized Least Squares
Fitting a Brownian Motion model in PGLS
Let's return to the Geospiza dataset (within the geiger package) to try PGLS. We assume that you have already loaded the necessary packages (geiger for the data and ape for the function) as described on this page. Let's say we want to test whether there is a significant relationship between wing length and tarsus length, accounting for possible dependence among the data points (trait values) due to phylogenetic relatedness.
First, let's prepare our tree for the analysis. Our original tree has 14 taxa but we only have data for 13, so we must prune the tree. We can take out the species for which we have no data (olivacea) as follows:
data(geospiza) attach(geospiza) geospiza13.tree<-drop.tip(geospiza.tree,"olivacea")
Now let's create a data frame containing just our traits of interest, with the row.names matching the tip.labels. Most users will bring their data in from a tab-delimited table, for example by typing:
where the row names are the taxon names. NOTE: you must associate the taxon names with the trait values so that the values can be correctly tied to the tips of the tree! See also this page. With the dataset in active memory, we can create the dataframe as follows:
wingL<-geospiza.data$wingL tarsusL<-geospiza.data$tarsusL DF.geospiza<-data.frame(wingL,tarsusL,row.names=geospiza13.tree$tip.label) DF.geospiza
When you type the final command, you will see that you have a data frame where the row names are the tip.labels in the pruned tree. Now we will first build the correlation structure expected if the traits evolve by Brownian motion and fit a generalized least squares model assuming this correlation structure.
bm.geospiza<-corBrownian(phy=geospiza13.tree) bm.gls<-gls(wingL~tarsusL,correlation=bm.geospiza,data=DF.geospiza) summary(bm.gls)
The summary shows you that the log likelihood of this model is 9.6 and its AIC score is -13.21. We also see that the effect of tarsus length on wing length is 1.37, and their relationship is signficant (p=0.001).
Fitting an Ornstein-Uhlenbeck Motion model in PGLS
Again we will first build the correlation structure, this time assuming if the traits evolve as expected under the Ornstein-Uhlenbeck process with a variance-restraining parameter, alpha. Ape automatically estimates the best fitting value of alpha for your data.
ou.geospiza<-corMartins(1,phy=geospiza13.tree) ou.gls<-gls(wingL~tarsusL,correlation=bm.geospiza,data=DF.geospiza) summary(ou.gls)
The summary shows you that the estimated alpha is 8.13, the log likelihood of this model is 10.73, and its AIC score is -13.47. This model estimates that the effect of tarsus length on wing length is 0.73, and their relationship is still significant (p=0.01).