Difference between revisions of "Family Alignment Documentation"

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(Analysis of Silent Substitutions Within a Genome or Across Multiple Genomes)
(Introduction)
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All of our examples are descriptions of bioinformatic workflows or pipelines. A workflow is comprised of a set of analytical applications, performing the analyses themselves, and some set of scripts that hand data to applications and take results from these same applications. One will also frequently encounter some set of critical filters (e.g. in English, "only write those sequences to a file that match the query sequence with a p < .0001" or "get all nucleotide sequences > 250 bp in length from the input file"), and these filtering steps may be performed by an application or by a script.
 
All of our examples are descriptions of bioinformatic workflows or pipelines. A workflow is comprised of a set of analytical applications, performing the analyses themselves, and some set of scripts that hand data to applications and take results from these same applications. One will also frequently encounter some set of critical filters (e.g. in English, "only write those sequences to a file that match the query sequence with a p < .0001" or "get all nucleotide sequences > 250 bp in length from the input file"), and these filtering steps may be performed by an application or by a script.
  
The characterized gene family, either as protein or nucleotide, is not necessarily an endpoint but is frequently the starting point for detailed phylogenetic analyses. For example one use case describes the analysis of the rate of silent substitutions within and between families. Another use case describes the task of comparing a species tree to gene family trees from those same species, and how one might resolve discrepancies between those structures.
+
The characterized gene family, either as protein or nucleotide, is not necessarily an endpoint but is frequently the starting point for detailed phylogenetic analyses. For example one use case describes the analysis of the rate of silent substitutions within and between families. Another use case describes the task of comparing a species tree to gene family trees from those same species, and how one might resolve discrepancies between those structures ([[Reconcile_Trees_Documentation|Reconcile Trees Documentation]]).
  
 
===Analysis of Gene & Protein Families from Fungal Genomes===
 
===Analysis of Gene & Protein Families from Fungal Genomes===
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* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=unigene UniGene]
 
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=unigene UniGene]
 
===Reconciling the Multiple Trees Created by Analyzing Genomes===
 
 
Based on the work of Stefanie Hartmann (http://visionlab.bio.unc.edu/).
 
 
====Applications====
 
 
* [http://www.cs.iastate.edu/~oeulenst/ Software from O. Eulenstein]
 
* [http://taxonomy.zoology.gla.ac.uk/rod/genetree/genetree.html GeneTree]
 
* [http://mesquiteproject.org/ Mesquite]
 
* [http://ginger.ucdavis.edu/gtp/gtp.html gtp]
 
* [http://prime.sbc.su.se/primetv/index.html primetv & reconcile]
 
* [http://www.cbu.uib.no/~steffpar/softparsmap/ softparsmap]
 
 
====Databases====
 
 
* [http://www.phytome.org www.phytome.org]
 
** Alignments and phylogenies for ~27,000 gene familes derived from 140 plant species
 

Revision as of 15:13, 12 December 2006

Introduction

These pages describe approaches to characterizing sequence families in some set of related organisms. There are many ways to do this so our examples, though robust and proven by practice, are selected from a multitude of possible examples. The focus is on approaches that use a Bio* toolkit ([BioJava, BioPerl, BioPython, BioRuby) since these packages offer the user different workflow possibilities and practical, re-useable code but home-grown solutions are also discussed.

All of our examples are descriptions of bioinformatic workflows or pipelines. A workflow is comprised of a set of analytical applications, performing the analyses themselves, and some set of scripts that hand data to applications and take results from these same applications. One will also frequently encounter some set of critical filters (e.g. in English, "only write those sequences to a file that match the query sequence with a p < .0001" or "get all nucleotide sequences > 250 bp in length from the input file"), and these filtering steps may be performed by an application or by a script.

The characterized gene family, either as protein or nucleotide, is not necessarily an endpoint but is frequently the starting point for detailed phylogenetic analyses. For example one use case describes the analysis of the rate of silent substitutions within and between families. Another use case describes the task of comparing a species tree to gene family trees from those same species, and how one might resolve discrepancies between those structures (Reconcile Trees Documentation).

Analysis of Gene & Protein Families from Fungal Genomes

Based on the work of Jason Stajich (http://fungal.genome.duke.edu/).

Applications

Databases

Analysis of Silent Substitutions Within a Genome or Across Genomes

Based on the work of Amy Bouck (http://visionlab.bio.unc.edu/).

Applications

Databases